Dr. No established most of the iconic traits of Bond :
"Bond, James Bond"
Honey Ryder coming out of the sea
Villain with Secret Lair
Exactly. In terms of "iconicity", Honey Ryder is undoubtedly one of the top images of franchise.
Dr No is no Blofeld, but he also left his iconic mark.
One more vote here for DN.
It might even be argued that it is the winner over GF even.
Or, it might be argued that it doesn't get to compete for having the unfair advantage of being the source. If that's the case, and I can't pick DN, I choose as runner-up...
That doesn't always make it the winner though. For example. Star Trek : The Motion Picture is pretty much forgotten these days, but Wrath of Khan became the iconic one. Ditto Rambo (II) being more iconic than First Blood (though Loomis will have to give that theory the official seal of approval!)
I'd say FRWL.
It has...
The Cold War
The best henchman ever (Red Grant)
An evil organization
Classic action scenes (boat chase, fight on train, shootout at gypsy camp)
Exotic locales.
There's nothing on this list that became as big a part of pop culture as Connery saying "Bond, James Bond" with the cigarette hanging out of his mouth or Honey Ryder coming out of the sea, though.
After GOLDFINGER, I'd say THE SPY WHO LOVED ME and DR NO have the most iconic images. But give it a few years and CASINO ROYALE will possibly fit in there.
Yeah CR has certainly made an impact in
modern pop culture, with Craig on the beach, the black and white opening, the second best ever "Bond, James Bond", the parkour scene, and just the whole surprise "Wow, they can actually make good Bond films these days?" public reception, but it'll be a few years before we can really judge how much of an impact it has/had.